Stapling peptides using cysteine crosslinking

Traceless ligation of cysteine peptides using selective deselenization.

Org . On Cysteine and Cystine Peptides . Part V . l S - Trityl - and S - Diphenyl - methyl - cysteine and - cysteine Peptides

A new method for the preparation of S-trityl(i.e. triphenylmethyl) and S-diphenylmethyl-cysteines or -cysteine peptides involves treatment of cysteine or cysteine peptides with aralkylcarbinols in the presence of trifluoroacetic acid or hydrogen halides in acetic acid solution. The optimal conditions for the removal of these S-protecting aralkyl groups by trifluoroacetic acid or hydrogen halide...

Interactions of AsCy3 with Cysteine-Rich Peptides

There is great interest in fluorogenic compounds that tag biomolecules within cells. Biarsenicals are fluorogenic compounds that become fluorescent upon binding four proximal Cys thiols, a tetracysteine (Cys(4)) motif. This work details interactions between the biarsenical AsCy3 and Cys(4) peptides. Maximal affinity was observed when two Cys-Cys pairs were separated by at least 8 amino acids; t...

A Perfluoroaryl-Cysteine S[subscript N]Ar Chemistry Approach to Unprotected Peptide Stapling

We report the discovery of a facile transformation between perfluoroaromatic molecules and a cysteine thiolate, which is arylated at room temperature. This new approach enabled us to selectively modify cysteine residues in unprotected peptides, providing access to variants containing rigid perfluoroaromatic staples. This stapling modification performed on a peptide sequence designed to bind the...

Obtaining secure stapling of a double stapling anastomosis.

BACKGROUND Anastomotic leakage is a serious complication after rectal surgery. The aim of this study was to assess the effect of waiting time during firing of stapling devices on optimal staple formation. METHODS An endoscopic linear stapler (Echelon Flex 60 Endopath) with either a 60 mm blue or gold cartridge was applied to the cardiac and pyloric portions of 27 fresh porcine stomachs. Three...